Results:
The markers-predictors of high efficacy of therapy with checkpoint inhibitors were identified and classified according to pathogenic mechanism of drug action:
1. High mutagenic potential of the tumor itself, characterized by the level of tumor mutation load (TMB), evolutionary divergence of HLA-I (HED), a high level of mutations in the circulating tumor DNA;
2. High activity of the adaptive immunity, which include low expression of inhibitory T-cell receptors, the presence of single nucleotide polymorphisms (SNPs) in genes encoding FcγR T-lymphocyte receptors, an increased number of tumor infiltrating lymphocytes (TILs), a high number of eosinophils and lymphocytes in peripheral blood (with predominance of CD8, CD4 memory T cells and decreased T-regulatory cells), high concentration of Il-2, VEGF-C and low concentration of Il-10;
3. Decreased activity of the tumor microenvironment, characterized by a reduced number of myeloid suppressor cells (MDSCs), relative decreased neutrophils in biopsy material and peripheral blood, low levels of Il-1β, Il-6, ANG2, C- reactive protein (CRP).
Conclusions: Determination of high rates (TMB), HED, high mutation rate in circulating tumor DNA before treatment can predict tumor response to the immune therapy, which can be used for more personalized patient selection. Biomarkers of innate and adaptive immunity may indicate the activity of tumor microenvironment and cytotoxic immunity. Determination of the markers before and during the treatment might be an appointment indication for various immunomodulatory drugs, inhibitors of pro-inflammatory cytokines and pro-angiogenic molecules.